Journal: Heliyon

Article Title: Identification of octyl gallate, a novel apoptosis-inducing compound for colon cancer therapy, from Sanguisorba officinalis L. by cell membrane chromatography and UHPLC-(Q)TOF-MS/MS

doi: 10.1016/j.heliyon.2024.e32230

Figure Lengend Snippet: The inhibitory impact of SO-MtOH on cell proliferation in SW620 cells. (A) The cellular viability of SW620 cells following a 48 h treatment with SO-MtOH was evaluated utilizing the MTT assay. (B) The viability of HCT116 cells following a 48 h treatment with SO-MtOH was assessed using the MTT assay to determine cell viability. (C) The cellular viability of SO-MtOH in DLD-1 cells following a 48 h treatment was quantified using the MTT assay. (D) The viability of CT26 cells following a 48 h treatment with SO-MtOH was assessed using the MTT assay to determine its impact on cell viability. The data reported in this study represents the mean ± standard deviation (SD) of six independent experiments. The data was analyzed using one-way analysis of variance (ANOVA) with Dunnett's 154 and two-way ANOVA with Tukey's 862 multiple comparisons test. The significance levels for the comparisons are denoted as follows: ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. control.

Article Snippet: L-O2 (human normal liver cell), HEK-293FT (human embryonic kidney 293FT), CCD841 CoN (human normal colonic epithelial cells), CT26 (mouse colon cancer cell CT26) and the colon cancer cell lines SW620, HCT116 and DLD-1 were provided by the China Center for Type Culture Collection (CCTCC, Wuhan, Hubei, China).

Techniques: MTT Assay, Standard Deviation, Control

Journal: Heliyon

Article Title: Identification of octyl gallate, a novel apoptosis-inducing compound for colon cancer therapy, from Sanguisorba officinalis L. by cell membrane chromatography and UHPLC-(Q)TOF-MS/MS

doi: 10.1016/j.heliyon.2024.e32230

Figure Lengend Snippet: The inhibitory impact of SO-MtOH on cell proliferation in SW620 cells. (A) The cellular viability of SW620 cells following a 48 h treatment with SO-MtOH was evaluated utilizing the MTT assay. (B) The viability of HCT116 cells following a 48 h treatment with SO-MtOH was assessed using the MTT assay to determine cell viability. (C) The cellular viability of SO-MtOH in DLD-1 cells following a 48 h treatment was quantified using the MTT assay. (D) The viability of CT26 cells following a 48 h treatment with SO-MtOH was assessed using the MTT assay to determine its impact on cell viability. The data reported in this study represents the mean ± standard deviation (SD) of six independent experiments. The data was analyzed using one-way analysis of variance (ANOVA) with Dunnett's 154 and two-way ANOVA with Tukey's 862 multiple comparisons test. The significance levels for the comparisons are denoted as follows: ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. control.

Article Snippet: L-O2 (human normal liver cell), HEK-293FT (human embryonic kidney 293FT), CCD841 CoN (human normal colonic epithelial cells), CT26 (mouse colon cancer cell CT26) and the colon cancer cell lines SW620, HCT116 and DLD-1 were provided by the China Center for Type Culture Collection (CCTCC, Wuhan, Hubei, China).

Techniques: MTT Assay, Standard Deviation, Control

Journal: Heliyon

Article Title: Identification of octyl gallate, a novel apoptosis-inducing compound for colon cancer therapy, from Sanguisorba officinalis L. by cell membrane chromatography and UHPLC-(Q)TOF-MS/MS

doi: 10.1016/j.heliyon.2024.e32230

Figure Lengend Snippet: Anti-proliferation effect of OG. ( A ) The cell viability of BAF (ZYG I, ZYG II, OG) in SW620 cells for 72 h treatment (mean ± S.D., n = 4); PTX as a positive control. ( B, C ) The cellular viability of SW620 and HCT116 after the 48 h treatment of OG, respectively (mean ± S.D., n = 4). ( D, E ) Cells were subjected to incubation with a combination of Hochest 33,342 and PI to capture cells with fluorescent signal under a fluorescence microscope. A bar chart depicting the comparative density of PI to hochest33342. ( F, G, H ) The impact of OG on cell viability of L-O2, HEK293FT and HUVEC cells (mean ± S.D., n = 4). The data reported in this study represents the mean ± standard deviation (SD) of four independent experiments. The data was analyzed using one-way analysis of variance (ANOVA) with Dunnett's 154 and two-way ANOVA with Tukey's 862 multiple comparisons test. The significance levels for the comparisons are denoted as follows: ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. control.

Article Snippet: L-O2 (human normal liver cell), HEK-293FT (human embryonic kidney 293FT), CCD841 CoN (human normal colonic epithelial cells), CT26 (mouse colon cancer cell CT26) and the colon cancer cell lines SW620, HCT116 and DLD-1 were provided by the China Center for Type Culture Collection (CCTCC, Wuhan, Hubei, China).

Techniques: Positive Control, Incubation, Fluorescence, Microscopy, Standard Deviation, Control

Journal: Heliyon

Article Title: Identification of octyl gallate, a novel apoptosis-inducing compound for colon cancer therapy, from Sanguisorba officinalis L. by cell membrane chromatography and UHPLC-(Q)TOF-MS/MS

doi: 10.1016/j.heliyon.2024.e32230

Figure Lengend Snippet: OG induces apoptosis in SW620 and HCT116 cells. The SW620 and HCT116 cell lines were subjected to treatment with varying concentrations of OG (0, 10, 20, 40, 80 μM) and a fixed concentration of PTX (40 nM) for a duration of 48 h. ( A, B ) The cells were treated with trypsin and subsequently cultured with a combination of Annexin V - FITC and PI, followed by flow cytometric analysis. A graphical representation in the form of a bar chart illustrating the rate of apoptosis in a biological system. ( C, D, E, F ) The cellular protein was subsequently extracted to investigate the presence of caspase-3, caspase-9, Bax, Bcl-2, and β-actin using western blotting analysis. The bar chart depicts the comparative densities of cleaved-caspase-3 to pro-caspase-3, cleaved-caspase-9 to pro-caspase-9, and Bax to Bcl-2. The data reported in this study represents the mean ± standard deviation (SD) of three independent experiments. The data was analyzed using one-way analysis of variance (ANOVA) with Dunnett's 154 and two-way ANOVA with Tukey's 862 multiple comparisons test. The significance levels for the comparisons are denoted as follows: ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. control.

Article Snippet: L-O2 (human normal liver cell), HEK-293FT (human embryonic kidney 293FT), CCD841 CoN (human normal colonic epithelial cells), CT26 (mouse colon cancer cell CT26) and the colon cancer cell lines SW620, HCT116 and DLD-1 were provided by the China Center for Type Culture Collection (CCTCC, Wuhan, Hubei, China).

Techniques: Concentration Assay, Cell Culture, Western Blot, Standard Deviation, Control

Journal: Heliyon

Article Title: Identification of octyl gallate, a novel apoptosis-inducing compound for colon cancer therapy, from Sanguisorba officinalis L. by cell membrane chromatography and UHPLC-(Q)TOF-MS/MS

doi: 10.1016/j.heliyon.2024.e32230

Figure Lengend Snippet: OG demonstrates a capacity to suppress the migration and invasion of SW620 and HCT116 cells. SW620 and HCT116 cells were treated with OG (0, 10, 20, 40 μM) and PTX (40 nM) for 24 h. ( A, B ) The wound-healing analysis demonstrated the migratory capacity of SW620 and HCT116 cells. A bar chart illustrating the migratory distance of SW620 and HCT116 cells. ( C, D ) Transwell assay was utilized to assess the invasive properties of SW620 and HCT116 cells. The bar chart depicted illustrates the invasion activity of SW620 and HCT116 cells. The data reported in this study represents the mean ± standard deviation (SD) of three independent experiments. The data was analyzed using one-way analysis of variance (ANOVA) with Dunnett's 154 and two-way ANOVA with Tukey's 862 multiple comparisons test. The significance levels for the comparisons are denoted as follows: ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. control.

Article Snippet: L-O2 (human normal liver cell), HEK-293FT (human embryonic kidney 293FT), CCD841 CoN (human normal colonic epithelial cells), CT26 (mouse colon cancer cell CT26) and the colon cancer cell lines SW620, HCT116 and DLD-1 were provided by the China Center for Type Culture Collection (CCTCC, Wuhan, Hubei, China).

Techniques: Migration, Transwell Assay, Activity Assay, Standard Deviation, Control